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geldoc ez imaging system  (Bio-Rad)


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    Structured Review

    Bio-Rad geldoc ez imaging system
    Geldoc Ez Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 4790 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/geldoc+imaging+system/Gel+Doc+EZ+System/bio_rxiv__64898__2026__02__15__705970-97-10-14
    Average 97 stars, based on 4790 article reviews
    geldoc ez imaging system - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Ruminococcus torques is a keystone degrader of intestinal mucin glycoprotein, releasing oligosaccharides used by Bacteroides thetaiotaomicron
    Article Snippet: Samples were collected, diluted 1:4 in Laemmli sample buffer (Bio-Rad), and heated for 10 min at 98°C. .. Samples were loaded onto 4%–12% Bis-Tris gradient gels (Thermo Fisher) unless otherwise noted and run for 60 min at 180 V. Gels were stained with periodic acid-Schiff stain as previously described ( ) and imaged using Bio-Rad GelDoc Imaging System. ..

    Article Title: Characterization of SLA RNA promoter from dengue virus and its interaction with the viral non-structural NS5 protein.
    Article Snippet: The Dengue virus (DENV) is the most significant arthropod-borne viral pathogen in humans with 400 million infections annually.. DENV comprises four distinct serotypes (DENV-1 to -4) which complicates vaccine development.. Any of the four serotypes can cause clinical illness but with distinctive infection dynamics.

    Imaging:

    Article Title: Ruminococcus torques is a keystone degrader of intestinal mucin glycoprotein, releasing oligosaccharides used by Bacteroides thetaiotaomicron
    Article Snippet: Samples were collected, diluted 1:4 in Laemmli sample buffer (Bio-Rad), and heated for 10 min at 98°C. .. Samples were loaded onto 4%–12% Bis-Tris gradient gels (Thermo Fisher) unless otherwise noted and run for 60 min at 180 V. Gels were stained with periodic acid-Schiff stain as previously described ( ) and imaged using Bio-Rad GelDoc Imaging System. ..

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2.
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). .. The relative protein expression levels were normalized with GAPDH as the internal reference of total protein and Lamin B as the internal reference of nuclear protein. qRT‐PCR Total RNA was extracted from cells using Trizol (Invitrogen life technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA by reverse transcription kit.

    Article Title: Characterization of SLA RNA promoter from dengue virus and its interaction with the viral non-structural NS5 protein.
    Article Snippet: The Dengue virus (DENV) is the most significant arthropod-borne viral pathogen in humans with 400 million infections annually.. DENV comprises four distinct serotypes (DENV-1 to -4) which complicates vaccine development.. Any of the four serotypes can cause clinical illness but with distinctive infection dynamics.

    Article Title: Phosphorylation of the nuclear poly(A) binding protein (PABPN1) during mitosis protects mRNA from hyperadenylation and maintains transcriptome dynamics
    Article Snippet: Total RNA was stained with Ponceau S (Thermo Scientific) as recommended by the manufacturer. .. Western blots were developed with ECL Western Blotting Detection Kit (Cytiva Amersham) and visualized using a Bio-Rad GelDoc imaging system. .. Cells were grown on No 1.5 coverslips (Zeiss) or 6 well plates for 24 h. Cells were then fixed in 4% paraformaldehyde, permeabilized in 0.25% Triton-X100 (American Bioanalytical) in PBS, blocked in 3% Bovine Serum Albumin (Sigma), and probed with the following antibodies in blocking buffer: Primary antibodies: mouse anti-SC35 and rabbit anti-PABPN1; Secondary antibodies: anti-rabbit Alexa-594 and anti-mouse Alexa-488 (Jackson laboratories).

    Article Title: MARCH family E3 ubiquitin ligases selectively target and degrade cadherin family proteins
    Article Snippet: Blots were rinsed and washed, incubated with Clarity ECL reagent (Bio-Rad Laboratories), and then imaged using a ChemiDoc MP system (Bio-Rad Laboratories). .. Band density was measured using a Bio-Rad GelDoc imaging system (Bio-Rad Laboratories) and Image Lab analysis software (Bio-Rad Laboratories). ..

    Article Title: Pulmonary tuberculosis epidemiology and genetics in Kazakhstan
    Article Snippet: .. Gel was visualized on a GelDoc imaging system (Bio-Rad, United States). ..

    Article Title: Dishevelled Segment Polarity Protein 3 (DVL3) Induced by Bacterial LPS Promotes the Proliferation and Migration of Prostate Cancer Cells through the TLR4 Pathway.
    Article Snippet: Background: Chronic inflammation is associated with various malignant tumors.. Bacterial lipopolysaccharides (LPSs) play a significant part in the event and development of prostate cancer.. Dishevelled segment polarity protein 3 (DVL3) is a shared component of the Wnt/β-catenin and Notch signaling pathways, which are involved in tumor progression, chemoresistance, and maintenance of stem cell-like properties.

    Article Title: Hemin as a protective agent in an in vitro model of hypoxia/reoxygenation-induced injury
    Article Snippet: Membranes were washed thrice with tris-buffered saline (TBST) to remove unbound primary antibodies, followed by a 2-h incubation with HRP secondary antibodies (Solarbio, Beijing, China) at room temperature. .. Protein bands were visualized using ECL reagent under the GelDoc imaging system (Bio-Rad no. 170-5060). β-actin served as the internal reference protein for normalizing protein expression levels. ..

    Membrane:

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2.
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). .. The relative protein expression levels were normalized with GAPDH as the internal reference of total protein and Lamin B as the internal reference of nuclear protein. qRT‐PCR Total RNA was extracted from cells using Trizol (Invitrogen life technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA by reverse transcription kit.

    Article Title: Dishevelled Segment Polarity Protein 3 (DVL3) Induced by Bacterial LPS Promotes the Proliferation and Migration of Prostate Cancer Cells through the TLR4 Pathway.
    Article Snippet: Background: Chronic inflammation is associated with various malignant tumors.. Bacterial lipopolysaccharides (LPSs) play a significant part in the event and development of prostate cancer.. Dishevelled segment polarity protein 3 (DVL3) is a shared component of the Wnt/β-catenin and Notch signaling pathways, which are involved in tumor progression, chemoresistance, and maintenance of stem cell-like properties.

    Incubation:

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2.
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). .. The relative protein expression levels were normalized with GAPDH as the internal reference of total protein and Lamin B as the internal reference of nuclear protein. qRT‐PCR Total RNA was extracted from cells using Trizol (Invitrogen life technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA by reverse transcription kit.

    Labeling:

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2.
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). .. The relative protein expression levels were normalized with GAPDH as the internal reference of total protein and Lamin B as the internal reference of nuclear protein. qRT‐PCR Total RNA was extracted from cells using Trizol (Invitrogen life technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA by reverse transcription kit.

    Agarose Gel Electrophoresis:

    Article Title: Characterization of SLA RNA promoter from dengue virus and its interaction with the viral non-structural NS5 protein.
    Article Snippet: The Dengue virus (DENV) is the most significant arthropod-borne viral pathogen in humans with 400 million infections annually.. DENV comprises four distinct serotypes (DENV-1 to -4) which complicates vaccine development.. Any of the four serotypes can cause clinical illness but with distinctive infection dynamics.

    Western Blot:

    Article Title: Phosphorylation of the nuclear poly(A) binding protein (PABPN1) during mitosis protects mRNA from hyperadenylation and maintains transcriptome dynamics
    Article Snippet: Total RNA was stained with Ponceau S (Thermo Scientific) as recommended by the manufacturer. .. Western blots were developed with ECL Western Blotting Detection Kit (Cytiva Amersham) and visualized using a Bio-Rad GelDoc imaging system. .. Cells were grown on No 1.5 coverslips (Zeiss) or 6 well plates for 24 h. Cells were then fixed in 4% paraformaldehyde, permeabilized in 0.25% Triton-X100 (American Bioanalytical) in PBS, blocked in 3% Bovine Serum Albumin (Sigma), and probed with the following antibodies in blocking buffer: Primary antibodies: mouse anti-SC35 and rabbit anti-PABPN1; Secondary antibodies: anti-rabbit Alexa-594 and anti-mouse Alexa-488 (Jackson laboratories).

    Software:

    Article Title: MARCH family E3 ubiquitin ligases selectively target and degrade cadherin family proteins
    Article Snippet: Blots were rinsed and washed, incubated with Clarity ECL reagent (Bio-Rad Laboratories), and then imaged using a ChemiDoc MP system (Bio-Rad Laboratories). .. Band density was measured using a Bio-Rad GelDoc imaging system (Bio-Rad Laboratories) and Image Lab analysis software (Bio-Rad Laboratories). ..

    Expressing:

    Article Title: Hemin as a protective agent in an in vitro model of hypoxia/reoxygenation-induced injury
    Article Snippet: Membranes were washed thrice with tris-buffered saline (TBST) to remove unbound primary antibodies, followed by a 2-h incubation with HRP secondary antibodies (Solarbio, Beijing, China) at room temperature. .. Protein bands were visualized using ECL reagent under the GelDoc imaging system (Bio-Rad no. 170-5060). β-actin served as the internal reference protein for normalizing protein expression levels. ..



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